Meili Wu1,2, Juyun He3, Karthik Masagounder4, Feng Huang2, Hongwei Liang1, Lixue Dong1, Hua Wen1, Ming Jiang1, Xing Lu1, Shengyan Su5, Juan Tian1
* Shengyan Su ouhaicourse@hotmail.com
* Juan Tian tianjuan@yfi.ac.cn
1 Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture and Rural Affairs, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China
2 Key Laboratory for Animal Nutrition and Feed Science of Hubei Province, Wuhan Polytechnic University, Wuhan 430023, China
3 School of Pharmaceutical Sciences, Guangzhou Medical University, Guangzhou 511436, China
4 Evonik Operations GmbH, Hanau‑Wolfgang 63457, Germany
5 Key Laboratory of Integrated Rice‑Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China
Abstract
This study evaluated the optimal DL-methionyl-DL-methionine (AQUAVI® Met-Met) supplementation for the red swamp crayfish Procambarus clarkii (initial body weight: 6.45 ± 0.12 g) fed plant-based diets. Seven diets were formulated, including a positive control with 50.0 g kg−1 fishmeal (PC), a negative control with 77.6 g kg−1 peanut meal replacing 50.0 g kg−1 fishmeal (NC), and five experimental diets supplemented with 0.25, 0.50, 1.00, 2.00, and 4.00 g kg−1 Met-Met. The analyzed Met-Met levels of the seven diets were 0.06 (PC), 0.10 (NC), 0.27 (M0.27), 0.46 (M0.46), 1.00 (M1.00), 1.99 (M1.99), and 4.10 g kg−1 (M4.10), respectively. After a 6-week feeding, crayfish fed the NC diet had lower growth performance, protein deposition, digestive enzyme activities, and antioxidant capacity than those fed the PC diet (P < 0.05). With the increasing Met-Met level to 1.00 g kg−1 in NC diet, the weight gain rate; specific growth rate; protein deposition rate; the activities of protease, lipase, and amylase in hepatopancreas and intestine; total antioxidant capacity; catalase and superoxide dismutase activities; and S-adenosyl homocysteine contents showed significant improvement (P < 0.05). This means the supplementation of 1.00 g kg−1 Met-Met in the non-fishmeal diet could alleviate the negative effects caused by all-plant protein formulation. Based on the broken-line regression analysis of weight gain rate and protein deposition rate in red swamp crayfish, the optimal levels of Met-Met in an all-plant protein diet were 0.90 and 0.95 g kg−1, and the levels of the total sulfur amino acid were 11.61 and 11.67 g kg−1, respectively.
This article was published in: Aquaculture International
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